说明书：

MF090- M5 GFP Rabbit Polyclonal Antibody.pdf

【BACKGROUND】

The green fluorescent protein (GFP) was originally identified as a protein involved in the bioluminescence of the jellyfish Aequorea victoria. GFP cDNA produces a fluorescent product when expressed in prokaryotic cells, without the need for exogenous substrates or cofactors, making GFP a useful tool for monitoring gene expression and protein localization in vivo. Several GFP mutants have been developed, including EGFP, which fluoresce more intensely than the wildtype GFP and have shifted excitation maxima, making them useful for FACS and fluorescence microscopy as well as double-labeling applications. GFP is widely used in expression vectors as a fusion protein tag, allowing expression and monitoring of heterologous proteins fused to GFP.

【REFERENCES】

1．Prasher, D.C., et al. 1992. Primary structure of the Aequorea victoria green fluorescent protein. Gene 111: 229-233.

2．Chalfie, M., et al. 1994. Green fluorescent protein as a marker for gene expression. Science 263: 802-805.

【SOURCE】

This polyclonal antibody is produced by immunizing animals with a synthetic peptide (KLH-coupled) corresponding to the carboxy terminus of EGFP.

【SPECIFICITY】

GFP Antibody detects GFP and GFP fusion proteins.

【REACTIVITY】

All

【ISOTYPE】

Rabbit IgG

【IMPORTANT】

Use an anti-RABBIT secondary antibody to detect the GFP antibody.

【RECOMMENDED ANTIBODY DILUTIONS】

Western blotting            1:5000

Immunofluorescence        1:2000