说明书：

MF085- M5 DYKDDDDK-Tag(3B9)mAb.pdf

【STORAGE】

Store at -20°C. Stable for one year from the date of shipment.

【BACKGROUND】

Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining        techniques. Due to their small size, they are unlikely to affect the tagged protein's biochemical properties. The DYKDDDDK peptide  has been used extensively as a general epitope tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion.

【REFERENCES】

 1.Brizzard, B. L. et al. 1994. Immunoaffinity purification of FLAG epitope-tagged bacterial alkaline phosphatase using    a novel monoclonal antibody and peptide elution. Biotechniques 16, 730-735.

【SOURCE】

  This monoclonal antibody is produced by immunizing animals with a synthetic DYKDDDDK peptide (KLH-coupled).

【SPECIFICITY】

  DYKDDDDK Tag Antibody detects exogenously expressed DYKDDDDK proteins in cells.

【REACTIVITY】

All

【ISOTYPE】

Mouse IgG2b

IMPORTANT

  Use an anti-Mouse secondary antibody to detect the 3B9 antibody.

RECOMMENDED ANTIBODY DILUTIONS

Western blotting            1:5000

Immunofluorescence        1:1000

Immunoprecipitation         1:100

ELISA                     1:2000

For Western blots, incubate membrane with diluted antibody in 5﹪w/v nonfat dry milk,1X TBS,0.05﹪Tween-20 at 4˚C with gentle shaking,overnight.